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KMID : 1103720110640010057
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Journal of the Korean Society of Radiology
2011 Volume.64 No. 1 p.57 ~ p.65
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A Bone Metastasis Nude Mouse Model Created by Ultrasound Guided Intracardiac Injection of Breast Cancer Cells: the Micro-CT, MRI and Bioluminescence Imaging Analysis
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Park Young-Jin
Song Eun-Hye
Kim Seol-Hwa
Choi Sang-Hyun
Song Ho-Taek
Suh Jin-Suck
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Abstract
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Purpose: The purpose of this study was to develop a nude mouse model of bone metastasis by performing intracardiac injection of breast cancer cells under ultrasonography guidance and we wanted to evaluate the development and the distribution of metastasis in vivo using micro-CT, MRI and bioluminescence imaging.
Materials and Methods: Animal experiments were performed in 6-week-old female nude mice. The animals underwent left ventricular injection of 2×105 MDA-MB-231Bo-Luc cells. After injection of the tumor cells, serial bioluminescence imaging was performed for 7 weeks. The findings of micro-CT, MRI and the histology were correlated with the ‘hot’ lesions seen on the bioluminescence imaging.
Results: Metastasis was found in 62.3% of the animals. Two weeks after intracardiac injection, metastasis to the brain, spine and femur was detected with bioluminescence imaging with an increasing intensity by week 7. Micro-CT scan confirmed multiple osteolytic lesions at the femur, spine and skull. MRI and the histology were able to show metastasis in the brain and extraskeletal metastasis around the femur.
Conclusion: The intracardiac injection of cancer cells under ultrasonography guidance is a safe and highly reproducible method to produce bone metastasis in nude mice. This bone metastasis nude mouse model will be useful to study the mechanism of bone metastasis and to validate new therapeutics.
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KEYWORD
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Breast Neoplasm, Disease Model, Animal, Luminescent Measurement, Magnetic Resonance Imaging, Neoplasms Metastasis
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